4 Conclusion

VCFshiny provides an easy-to-use framework for annotating and statistical analysis of VCF(variant call format) variant data after high-throughput sequencing analysis. VCFshiny can analyze a variety of data types from multiple perspectives, and provide annotation and a variety of analysis and visualization methods for variable data. By analyzing and testing test data, we believe that VCFshiny can be a very useful tool in the field of variation analysis.